Hdac4 Enhances Synovium-derived Stem Cell-based Chondrogenesis Induced by Tgf-β1
ثبت نشده
چکیده
Introduction Transforming growth factor beta (TGF-β) superfamily members play diverse roles in cartilage development and maintenance. TGF-β not only up-regulates a number of molecules associated with prechondrogenic condensation, it also up-regulates chondrogenic gene expression through several signaling pathways. Moreover, TGF-β has been reported to inhibit osteoblast differentiation by repressing runt-related transcription factor 2 (Runx 2) . However, our previous study indicated that TGF-β1 alone is not sufficient to fully differentiate mesenchymal stem cells (MSCs) into chondrocytes . Other factors are necessary for the chondrogenic differentiation process. Histone deacetylases (HDACs) are the enzymes catalyzing the deacetylation of histone and non-histone proteins and regulating cell proliferation, differentiation, and apoptosis. Recently, it was reported that HDAC4 acts as a negative regulator of chondrocyte hypertrophy . We hypothesize that HDAC4 may promote TGF-β1-induced stem cell-based chondrogenesis.
منابع مشابه
Induction of Chondrogenic Differentiation of Human Adipose-Derived Stem Cells with TGF-β3 in Pellet Culture System
Objective Adult stem cells which are derived from different tissues, with their unique abilities to self-renew and differentiate into various phenotypes have the potential for cell therapy and tissue engineering. Human adipose tissue is an appropriate source of mesenchymal stem cells with wide differentiation potential for tissue engineering research. In this study isolated stem cells from hum...
متن کاملTGF-β1 conjugated chitosan collagen hydrogels induce chondrogenic differentiation of human synovium-derived stem cells
BACKGROUND Unlike bone tissue, articular cartilage regeneration has not been very successful and has many challenges ahead. We have previously developed injectable hydrogels using photopolymerizable chitosan (MeGC) that supported growth of chondrocytes. In this study, we demonstrate a biofunctional hydrogel for specific use in cartilage regeneration by conjugating transforming growth factor-β1 ...
متن کاملDifferential effects of dexamethasone on the chondrogenesis of mesenchymal stromal cells: influence of microenvironment, tissue origin and growth factor.
Mesenchymal stromal cells (MSCs), which reside within various tissues, are utilized in the engineering of cartilage tissue. Dexamethasone (DEX)--a synthetic glucocorticoid--is almost invariably applied to potentiate the growth-factor-induced chondrogenesis of MSCs in vitro, albeit that this effect has been experimentally demonstrated only for transforming-growth-factor-beta (TGF-β)-stimulated b...
متن کاملComparison of the efficacy of Piascledine and transforming growth factor β1 on chondrogenic differentiation of human adipose-derived stem cells in fibrin and fibrin-alginate scaffolds
Objective(s):The aim of this study was to compare the chondrogenic induction potential of Piascledine and TGF-β1 on adipose-derived stem cells (ADSCs) in fibrin and fibrin-alginate scaffolds. Materials and Methods: Human subcutaneous adipose tissues were harvested from three patients who were scheduled to undergo liposuction. Isolated ADSCs were proliferated in a culture medium. Then, the cell...
متن کاملSmall Molecule-BIO Accelerates and Enhances Marrow-Derived Mesenchymal Stem Cell in Vitro Chondrogenesis
Background: Hyaline cartilage defects exhibit a major challenge in the field of orthopedic surgery owing to its limited repair capacity. On the other hand, mesenchymal stem cells (MSCs) are regarded as potent cells with a property of cartilage regeneration. We aimed to optimize marrow-derived MSC chondrogenic culture using a small bioactive molecule referred to as BIO. Methods: MSCs from the ma...
متن کامل